9d. Why procedures were done
What to write
For each experimental group, including controls, describe why procedures were conducted.
Explanation
There may be numerous approaches to investigate any given research problem; therefore, it is important to explain why a particular procedure or technique was chosen. This is especially relevant when procedures are novel or specific to a research laboratory or constrained by the animal model or experimental equipment (e.g., route of administration determined by animal size1).
Examples
‘Because of the very small caliber of the murine tail veins, partial paravenous injection is common if 18F-FDG is administered by tail vein injection (intravenous). This could have significantly biased our comparison of the biodistribution of 18F-FDG under various conditions. Therefore, we used intraperitoneal injection of 18F-FDG for our experiments evaluating the influence of animal handling on 18F-FDG biodistribution’2.
‘Since Xenopus oocytes have a higher potential for homologous recombination than fertilized embryos… we next tested whether the host transfer method could be used for efficient HDR-mediated knock-in. We targeted the C-terminus of X. laevis Ctnnb1 (β-catenin), a key cytoskeletal protein and effector of the canonical Wnt pathway, because previous studies have shown that addition of epitope tags to the C-terminus do not affect the function of the resulting fusion protein (Fig …). CRISPR components were injected into X. laevis oocytes followed by host transfer or into embryos’3.
Training
The UK EQUATOR Centre runs training on how to write using reporting guidelines.
Discuss this item
Visit this items’ discussion page to ask questions and give feedback.
